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KMID : 0380020070220020084
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Korean Journal of Biotechnology and Bioengineering
2007 Volume.22 No. 2 p.84 ~ p.90
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Flow Injection Analysis for On-line Monitoring of Trehalose in Fermentation Processes
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Han Kyung-Ah
Lee Jong-ll
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Abstract
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Trehalose is non-reducing disaccharide which is found in bacteria, fungi, plants and insects. Trehalose has been determined by several analysis methods. To monitor the concentrations of trehalose in a process, enzymatic methods have more advantage over others, e.g. more specific. In this work, trehalase was immobilized on VA-epoxy polymer and applied to FIA systems. The behaviours of these FIA systems were characterized and used to monitor the trehalose concentrations. Use of optical detection technique was chosen for trehalose-FIA system. On-line monitoring data and off-line data were measured by HPLC.
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KEYWORD
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Flow injection analysis, trehalose, on-Line monitoring, fermentation process
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